fig5

Figure 5. Cardiomyocyte contractile and intracellular Ca²⁺ properties in fetal (D135) hearts from ewes receiving either control or overnutrition diet (150% NRC recommendation) for 135 days. (A) Resting cell length; (B) PS; (C) +dL/dt; (D) -dL/dt; (E) TPS; (F) TR₉₀; (G) Baseline intracellular Ca²⁺ FFI; (H) ΔFFI; and (I) Intracellular Ca²⁺ decay constant. Mean ± SEM, *P < 0.05 between indicated groups, n = 23 (A-F) or 22 (G-I) cells (technical replicates) from 3 fetuses (biological replicates) per group.