fig7

Figure 7. Protein levels of proinflammatory and ferroptotic markers in fetal (D135) hearts from ewes receiving either control or overnutrition (150% NRC recommendation) for 135 days. (A) Representative immunoblots of CD14, CD68, IL1A (IL1α), IL1B (IL1β), IL6, IL18, TLR4, iNOS, M-CSF, GPX4, and SLC7A11 using specific antibodies (using GAPDH or α-tubulin as loading controls);(B) CD14 protein level; (C) CD68 protein level; (D) IL1A (IL1α) protein level; (E) IL1B (IL1β) protein level; (F) IL6 protein level; (G) IL18 protein level; (H) TLR4 protein level; (I) iNOS protein level; (J) M-CSF protein level; (K) GPX4 protein level; (L) SLC7A11 protein level; (M) NFκB level; and (N) phosphorylated IκB (pIκB)-to-IκB ratio. Insets: Representative immunoblots of NFκB, pIκB, and IκB using specific antibodies (GAPDH as the loading control). Mean ± SEM, *P < 0.05 between groups, n = 6-7 fetuses per group.