fig6

From: Hepatic Smad4 mediates antibacterial defense via macrophage recruitment

Figure 6. Smad4 regulate the expression of Reg3b and Reg3g via Stat3 and GR.
(A) ChIP assay using Smad4 antibody followed by qPCR of the Reg3b promoter (SMAD4-BE1, SMAD4-BE2) in the liver of non-infected control mice, E. coli infected control mice and E. coli infected LKO mice (n = 4 per group); (B) ChIP assay using Smad4 antibody followed by qPCR of the Reg3g promoter (SMAD4-BE1, SMAD4-BE2 and SMAD4-BE3) in the liver of non-infected control mice, E. coli infected control mice and E. coli infected LKO mice (n = 4 per group). (C) Stat3 ChIP followed by qPCR of the Reg3b promoter (STAT3-BE1 and STAT3-BE2) in the liver of non-infected control mice, E. coli infected control mice and E. coli infected LKO mice (n = 4 per group); (D) Stat3 ChIP followed by qPCR of the Reg3g promoter (STAT3-BE1, STAT3-BE2, STAT3-BE3) in the liver of non-infected control mice, E. coli infected control mice and E. coli infected LKO mice (n = 4 per group). Circles correspond to individual mouse. Results are represented as mean ± SD. The P values in (A-D) were determined using one-way ANOVA. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. NS: Not significant; ChIP: chromatin immunoprecipitation; qPCR: quantitative polymerase chain reaction; E. coli: Escherichia coil; LKO: liver-specific Smad4 knockout; GR: glucocorticoid receptor.