fig4

Figure 4. Bilirubin inhibits SREBP activity in vitro. (A) SRE-Luc/HL-7702 cells were treated with bilirubin (12.5-100 μM) for 6 h before cell lysis and luciferase activity measurement; (B) Time-course analysis of SRE-Luc reporter activity in HL-7,702 cells treated with bilirubin (50 μM) for 2-16 h; (C-F) Immunoblot analysis of HL-7702 whole-cell lysates using specific antibodies; (G and H) Q-PCR analysis of gene expression changes in HL-7702 cells following bilirubin treatment (25/50 μM). All experiments were performed in biological triplicates. SREBP: Sterol regulatory element-binding proteins; SRE: sterol regulatory element.