fig5
Figure 5. Bilirubin inhibits the SREBP activity in HFD-fed mice. (A) Liver protein lysates (pooled from 3 mice/group) were immunoblotted with target-specific antibodies; (B) SREBP-1 expression levels were normalized to GAPDH for quantitative analysis; (C and D) Hepatic RNA extracts underwent Q-PCR quantification using mouse GAPDH as an endogenous control, per established protocols. Error bars represent standard deviations. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001 vs. HFD. SREBP: Sterol regulatory element-binding proteins; HFD: high-fat diet.