fig4

Figure 4. K. pneumoniae is unable to use intact mucus as a fuel source. K. pneumoniae ATCC 35657 (A and E), ATCC 700603 (B and F), CB1 (C and G), and MCFM22 (D and H) were grown in a M9 minimal medium supplemented with (A-D) porcine MUC2 (1, 0.5, and 0.25 mg/mL) or (E and H) human MUC2 (1, 0.5, and 0.25 mg/mL) for 18 h. The OD₆₀₀ was measured by plate reader to monitor growth over time. Data are represented as mean ± standard deviation. Graphs generated with Graphpad Prism. ATCC: American Type Culture Collection; OD₆₀₀: optical density at 600 nm; K. pneumoniae: Klebsiella pneumoniae; MUC2: Mucin 2.