fig2

Figure 2. Intracellular behavior of G/T@L-E. (A) Growth curves of E. coli DH5α and G/T@L-E. n = 3; (B) Cell viability of 4T1 cells following different treatments. n = 5; (C) CLSM images of 4T1 cells stained with live/dead after various treatments. Scale bar: 100 µm; (D) CLSM images of 4T1 cells incubated with different treatments for 4 h. Scale bar: 20 µm; (E) Relative viabilities of 4T1 cells after 24 h incubation with different treatments with or without glucose. n = 5; (F and G) CLSM images and the corresponding fluorescence intensity of DCFH-DA-stained 4T1 cells. n = 3. Scale bar: 100 µm. Data are presented as mean ± SD. Significance was compared by one-way ANOVA followed by Dunnett’s multiple comparisons test or two-way ANOVA followed by Tukey’s multiple comparisons test. ^**P < 0.01, ^****P < 0.0001. E. coli: Escherichia coli; CLSM: confocal laser scanning microscopy; DCFH-DA: 2,7-dichlorodihydrofluorescein diacetate; SD: standard deviation; ANOVA: analysis of variance; CFU: colony-forming unit; CAL: calcein acetoxymethyl; PI: propidium iodide; PBS: phosphate-buffered saline; FITC: fluorescein isothiocyanate.